以下为我的修改意见，请译者进一步参考：
However, understanding the regulatory processes of the humoral response to transplantation and the mechanisms underlying therapeutic agents and protocols for preventing and treating sensitization requires a way to study HLA-specific B cells.
但是，要了解针对移植的体液反应的调节过程、潜在治疗因子的机制、预防和治疗致敏（患者）方案，需要研究HLA特异性B细胞。
Lymphocyte preparations enriched for B cells were stained with one or more of three different HLA tetramers. Tetramer-positive (tet+) B cells were enumerated and evaluated for an association of their frequencies with known sensitization. In some cases, tet+ B cells were isolated and placed in culture with supplements known to activate B cells in a nonspecific fashion.
富含B细胞的淋巴细胞标本用三种不同的HLA 四聚体中的1种或多种进行染色。对四聚体阳性(即tet+)的B细胞进行计数，评价其频率与已知的致敏作用的相互关联。在部分实验中，四聚物阳性B细胞被分离出来并放置在含有可以非特异性方式激活B细胞的补体的培养基内。
RESULTS: For all tetramers used, the frequencies of tet+ B cells were significantly higher (4.1%-5.5%) among sensitized patients than among nonsensitized patients (1.6%-3.2%, P<0.001). Binding of the tetramers occurred by the surface immunoglobulin antigen receptor with little or no binding to antibody captured in the Fc receptor.
结果：对所应用的所有四聚物而言，四聚物阳性B细胞在致敏患者中的出现频率明显高(4.1%-5.5%)于非致敏患者(1.6%-3.2%, P<0.001)中的出现率。四聚物的结合是通过表面免疫球蛋白抗原受体，通过Fc受体结合抗体的很少量甚至没有。
Cultured tet+ B cells produced antibodies specific for epitopes of the tetramer antigen. There appeared to be a certain amount of crossreactivity in the binding of tetramers. The frequency of CD27+ cells among tet+ B cells was higher, on average (34.4%-38.8%) than among all B cells (26.2%) whereas the frequencies of CD38 were comparable in the two groups.
培养的四聚体阳性B细胞产生针对四聚体抗原表位的特异性抗体。这提示在结合四聚体的反应中出现了一定数量的交叉反应。四聚体阳性B细胞中CD27+细胞出现频率高于其在总B细胞中的频率，前者平均达34.4%-38.8%而在后者是26.2%。然而，CD38的表达率在两组细胞中基本相当。
CONCLUSIONS: Staining with HLA tetramers provides a means for identifying, quantifying, and isolating HLA-specific B cells.
结论：HLA四聚体染色法为HLA特异性B细胞的鉴别、定量、及分离提供了一种新的手段。
